Masixole was born in Uitenhage, Kwa-Nobuhle. He attended Solomon Mahlangu High School and went on to complete an honours degree in Political Science at Nelson Mandela Metropolitan University and a Masters degree in International and Political studies at Rhodes University. Masixole has been involved in student and community activism and served in different leadership positions. He was an active member of SASCO NMMU, Young Communist League, Culture Consciousness, Black Student Movement, FeesMustFall movement and Pan African Youth Dialogue. He also worked with workers, particularly shop stewards on campus at Rhodes University.